The Investigation of the Interaction between Human Serum Transferring with Colchicine in the Presence of Pb+2 Ions: Synchronous Fluorescence Measurements

Author

Abstract

The interaction between Holo-Transferin (HTF) and Colchicine (COL) was investigated in the present of Pb+2 ions under physiological conditions by using synchronous fluorescence spectra. The synchronous fluorescence spectra show a slight change of tryptophan residue micro-environment. Synchronous fluorescence spectra show that the structure of the tyrosine residue environment was altered by interaction of the COL and Pb+2 ions with HTF. The fluorescence intensity of HTF decreased regularly beside a small blue shift with increasing concentrations of COL and Pb+2 ions. The intrinsic fluorescence of HTF was quenched in the presence of drug and ion. Interaction of drugs with HTF and HTF-Pb+2 can elucidate the properties of drug-protein and ion- protein complex, as it may provide useful information about the structural feature that determines the therapeutic effectiveness of ion and drugs. Therefore, it has become a significant research field in life science, chemistry, biotechnology and clinical medicine.

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  1. Aisen P., A. Sigel and H. Sigel (Eds.), "Metals in
  2. Biological Systems", 35, Marcel Dekker, New
  3. York, (1998), 585ââ‚‌“665.
  4. Farce, A., C. Loge, S. Gallet, N. Lebegue, P.
  5. Carato, P. Chavatte, P. Berthelot and D.
  6. Lesieur, "Docking study of ligands into the
  7. colchicine binding site of jubulin", J. Enzym.
  8. Inhib. Med. Chem,, 19,(2004), 541-547.
  9. Jonsson P, "Anisotropy, disorder and frustration in
  10. magnetic nanoparticle system and spin glasses,
  11. Comprehensive Summaries of Uppsala
  12. Disertations from the Faculty of Science and
  13. Technology". 727, 2002
  14. Katrahalli U., S. Jaldappagari and S.S Kalanur,
  15. "Study of the interaction between fluoxetine
  16. hydrochloride and bovine serum albumin in the
  17. imitated physiological conditions by multispectroscopic
  18. methods", Journal of
  19. Luminescence , 130 (2010) 211-216.
  20. Lioyd J.B.F. and I.W. Evett, "Prediction of peak
  21. wavelengths and intensities in synchronously
  22. excited fluorescence emission spectra", Anal.
  23. Chem. 49 (1977) 1710ââ‚‌“1715.
  24. Metz-Boutigue M.-H., J. Jolles, J. Mazurier, F.
  25. Schoentgen, D. Legrand, G. Spik, J. Montreuil
  26. and P. Jollts, Eur and J. Biochem., 145 (1984)
  27. -676