Acute Genotoxic Effects of Effluent Water of Thermo-Power Plant “Kosova” In Tradescantia Pallida

Authors

Department of Biology, University of Prishtina, P.O.Box 10.000 Prishtinë, Republic of Kosovo

Abstract

The aim of this study was the evaluation of acute genotoxic effect of effluent water of thermo-power plant by means of Tradescantia root tips micronucleus test (MN), mitotic index and cell aberrations.   Tradescantia, was experimentally treated (for 24 h), with effluent water of thermo-power plant in different dilution ratios (negative control – distilled water; primary untreated effluent water and 1:1; 1:2; 1:3; 1:4; 1:5; 1:6 and 1:7 respectively). Number of aberrant cells, and frequency of micronuclei (MN), in meristematic root tip cells of treated plants (Tradescantia), were significantly increased (P<0.001; P<0.001 respectively), while the mitotic index in all treated plants was progressively decreased in comparison to the negative control. The results of present study indicate that Tradescantia root-tip micronucleus assay with direct exposure of intact plants is an appropriate method which enables to detect genotoxic effects of effluent waters.

Keywords

References

  1. De Rainho,C.R., A. Kaezner, C.A.F.Aiub, and I.
  2. Felzenszwalb.2010. Ability of Alium cepa L.
  3. root tips and Tradescantia pallida var.
  4. puerpurea in N-nitrosodiethylamine
  5. genotoxicity and mutagenicity evaluation. An
  6. Acad Bras Cience. 82, (4), 921-932.
  7. DeMarco A., S. Paglialunga, M. Rizzoni, A. Testa
  8. and S. Trinca. 1988. Induction of micronuclei
  9. in Vicia faba roots tips treated with heavy
  10. metals (cadmium and chromium) in the
  11. presence of NTA. Mutat. Res. 206, 311-315.
  12. Eckel, P.M. 1995. Aquatic genotoxicity testing with
  13. rat hepatocytes in primary culture II. Induction
  14. of micronuclei and chromosomal aberrations.
  15. Sci Total Environ. 159, 81-89.
  16. Egito L. C. M., M.G. Medeiros, S. R. B. Medeiros,
  17. and L.F. Agez-Lima. 2007. Cytotoxic and
  18. genotoxic potential of surface water from the
  19. Pitimbu river, northeastern /RN Brazil.
  20. Genetics and Molecular Biology, 30 (2), 435-
  21. Elezaj, I.R., K.H.Kurteshi, Q.I.Selimi, and
  22. K.Rr.Letaj. 2003. Genotoxic effects of Sitnica
  23. river (polluted from effluent waters of
  24. ââ‚‌œKosovaââ‚‌ Power plant) in three fish species
  25. (Barbus barbus, Perca fluviatilis and
  26. Oncorhynchus muciss Walbaum). FASEB.
  27. A1368/ 886.38.
  28. Haider, T., R. Sommer, S. Knasmuller, P. Eckel,
  29. W. Pribil, A. Cabaj and M. Kundi.2002.
  30. Genotoxic response of Austrian groundwater
  31. samples treated under standardized UV (254
  32. nm)- disinfection conditions in a comb ination
  33. of three different bioassays. Water Research.
  34. , 25-32.
  35. Helma C., S. Knasmuller , and R. Schulte Herman.
  36. Bie Belastung von Wassern mit
  37. genotoxischen Substanzen I. Methoden zur
  38. Prufung der Genotoxizita. Z. Umweltchem
  39. Okotox 6, 277-288.
  40. Ivanova, E., A. S. Teodora, and I. Velcheva. 2005.
  41. Cytogenetic testing of heavy metal and
  42. cyanide contaminated river waters in a mining
  43. region of Southwest Bulgaria. Journal of Cell
  44. and Molecular Biology. 4, 00-106.
  45. Knasmuller, S., E. Gottman, H. Steinkellner, A.
  46. Fomin, Pickl C, A. Paschke, R. God, and M.
  47. Kundi.1998. detection of genotoxic effects of
  48. heavy metal contamination soils with plant
  49. bioassays. Mutation Research., 2720; 000-000.
  50. Knasmuller, S., C. Helma, P. M. Eckel, E.
  51. Gottman, H. Steinkellner, F. Kassie, T. Haider,
  52. W.Parzefall, and R. Schulte-Hermann. 1998.
  53. Investigation on genotoxic effects of
  54. groundwater from the Mitterndorfer Senke and
  55. from the vicinity of Wiener Neustadt. 110, 23,
  56. -833.
  57. Kundi.1998. detection of genotoxic effects of heavy
  58. metal contamination soils with plant bioassays.
  59. Mutation Research., 2720;000-
  60. micronucleus (TradMCN) bioassay on
  61. clastogenicity of wastewater and in situ
  62. monitoring. Mutat. Res. 270, 45-51.
  63. Ma, T.H., 1982. Tradescantia cytogenetic tests
  64. (root-tip mitosis, pollen mitosis, pollen mother
  65. ââ‚‌“ cell meiosis). Mut. Res., 99, 293-302.
  66. Ma, T.H., Z. Xu, C. Xu, H. McConnell, E. V.
  67. Rabago, G.A. Arreola and H. Zang. 1995. The
  68. improved Allium/Vicia root tip micronucleus
  69. assay for clasto genicity of environmental
  70. pollutants. Mutation Research - Environmental
  71. Mutagenesis and related Subjects. 334 (2),
  72. -195.
  73. Majer, B., D. Tscherko, A. Paschke, R. Wennrich,
  74. M. Kundi, E. Kandellr and S. Knasmuller.
  75. Effects of heavy metal contamination of
  76. soils on micronucleus induction in Tradescantia
  77. and microbial enzyme activities: a
  78. comparative investigation. Mutation Research.
  79. , 111-124.
  80. Matsumoto, S.T., M.S. Mantovani, M. Irene, A.
  81. Malaguttii, A.L. Dias, I. C. Fonseca and M.A.
  82. Marin-Morales. 2006. Genotoxicity and
  83. mutagenicity of water contaminated with
  84. tannery effluents, as evaluated by
  85. micronucleus test and comet assay using fish
  86. Oreochromis niloticus and chromosome
  87. aberrations in onion root-tips. Genetics and
  88. Molecular Biology. 29 (1), 148-158
  89. Misk, M., T.H., Ma, A. Nersesyan, S. Monarca,
  90. J.K. Kim and S. Knasmuller. 2011.
  91. Micronucleus assays with the Tradescantia
  92. pollen tetrads: and update. Mutagenesis., 26
  93. (1), 215-221.
  94. Odeigah, P.G.C., O. Nurudeen, and O.O. Amund .
  95. Genotoxicity of oil field wastewater in
  96. Nigeria. Hereditas. 126, 161-167.
  97. Rizaj, M., Beqiri E, McBow, Oââ‚‌™Brein E.Z,
  98. Kongoli F, 2008. The mineral base and
  99. productive capacities of metals and nonmetals
  100. of Kosovo. JOM., vol.60 No.8:18-22.
  101. Ruiz, E. F., V. M. E. Rabago, S. U. Lecona, A. B.
  102. Perez and T. H. Ma. 1992. Tradescantia ââ‚‌“
  103. micronucleus (Trad-MCN) bioassay on clasto
  104. genicity of wastewater and in situ monitoring.
  105. Mutat. Res. 270, 45-51.
  106. Samka-Kinci, V., P. Stegnar, M. Lovka and M. J.
  107. Toman. 1996. The evaluation of waste,
  108. Surface and ground water quality using the
  109. Alium test procedure. Mutation Research/
  110. Genetic Toxicology. 368 (3-4), 171-179.
  111. Samuel , O.B., F.I. Osuala and P. G. C. Odeigah.
  112. Cytogenotoxicity evaluation of two
  113. industrial effluents using Allium cepa assay.
  114. African Journal of Environmental Science and
  115. Technology. 41 (1), 21-27.
  116. ZSandhu, S.S., T.H. MA, Y. Peng and H. Zhou.
  117. Clastogenicity of seven chemicals
  118. commonly found at industrial waste sites.
  119. Mutat. Res. 224, 437-445.
  120. Shokal R.R., and F.J. Rholf. 1981. Biometry, 2nd
  121. Edn., Freeman. New York, 1981, pp. 427-428.
  122. Steinkenller, H., F. Kassie, and S.
  123. Knasmuller.1999. Tradescantia ââ‚‌“ micronucleus
  124. assay for the assessment of the clastogenicity
  125. of Austrian water. Mutation Research. 426,
  126. -116.
  127. Tolbert, P. E., C. M. Shy, and J.W. Allen. 1992.
  128. Micronuclei and other ano- malies in buccal
  129. smears: methods Mutation Research, 271, 69-
  130. Zahn, R. K., H. C. Schroder, and H. G.
  131. Miltenburger. 1992

Files

History

  • Receive Date: 28 September 2011
  • Revise Date: 25 September 2021
  • Accept Date: 29 October 2018

Share

How to cite

Statistics