Home Articles List Article Information
Journal of Chemical Health Risks
Journal Archive
Volume Volume 9 (2019)
Volume Volume 8 (2018)
Volume Volume 7 (2017)
Volume Volume 6 (2016)
Volume Volume 5 (2015)
Volume Volume 4 (2014)
Volume Volume 3 (2013)
Volume Volume 2 (2012)
Volume Volume 1 (2011)
Elezaj, I., Millaku, L., Imeri-Millaku, R., Selimi, Q., Letaj, K. (2011). Acute Genotoxic Effects of Effluent Water of Thermo-Power Plant “Kosova” In Tradescantia Pallida. Journal of Chemical Health Risks, 1(1), -. doi: 10.22034/jchr.2011.543969
I.R. Elezaj; L.B. Millaku; R.H. Imeri-Millaku; Q.I. Selimi; K. Rr. Letaj. "Acute Genotoxic Effects of Effluent Water of Thermo-Power Plant “Kosova” In Tradescantia Pallida". Journal of Chemical Health Risks, 1, 1, 2011, -. doi: 10.22034/jchr.2011.543969
Elezaj, I., Millaku, L., Imeri-Millaku, R., Selimi, Q., Letaj, K. (2011). 'Acute Genotoxic Effects of Effluent Water of Thermo-Power Plant “Kosova” In Tradescantia Pallida', Journal of Chemical Health Risks, 1(1), pp. -. doi: 10.22034/jchr.2011.543969
Elezaj, I., Millaku, L., Imeri-Millaku, R., Selimi, Q., Letaj, K. Acute Genotoxic Effects of Effluent Water of Thermo-Power Plant “Kosova” In Tradescantia Pallida. Journal of Chemical Health Risks, 2011; 1(1): -. doi: 10.22034/jchr.2011.543969

Acute Genotoxic Effects of Effluent Water of Thermo-Power Plant “Kosova” In Tradescantia Pallida

Article 5, Volume 1, Issue 1, Summer 2011  XML PDF (49.44 K)
DOI: 10.22034/jchr.2011.543969
Authors
I.R. Elezaj email ; L.B. Millaku; R.H. Imeri-Millaku; Q.I. Selimi; K. Rr. Letaj
Department of Biology, University of Prishtina, P.O.Box 10.000 Prishtinë, Republic of Kosovo
Receive Date: 28 September 2011,  Revise Date: 20 June 2019,  Accept Date: 29 October 2018 
Abstract
The aim of this study was the evaluation of acute genotoxic effect of effluent water of thermo-power plant by means of Tradescantia root tips micronucleus test (MN), mitotic index and cell aberrations.   Tradescantia, was experimentally treated (for 24 h), with effluent water of thermo-power plant in different dilution ratios (negative control – distilled water; primary untreated effluent water and 1:1; 1:2; 1:3; 1:4; 1:5; 1:6 and 1:7 respectively). Number of aberrant cells, and frequency of micronuclei (MN), in meristematic root tip cells of treated plants (Tradescantia), were significantly increased (P<0.001; P<0.001 respectively), while the mitotic index in all treated plants was progressively decreased in comparison to the negative control. The results of present study indicate that Tradescantia root-tip micronucleus assay with direct exposure of intact plants is an appropriate method which enables to detect genotoxic effects of effluent waters.
Keywords
Tradescantia; MN-test; cell aberration; mitotic index
References
  1. De Rainho,C.R., A. Kaezner, C.A.F.Aiub, and I.
  2. Felzenszwalb.2010. Ability of Alium cepa L.
  3. root tips and Tradescantia pallida var.
  4. puerpurea in N-nitrosodiethylamine
  5. genotoxicity and mutagenicity evaluation. An
  6. Acad Bras Cience. 82, (4), 921-932.
  7. DeMarco A., S. Paglialunga, M. Rizzoni, A. Testa
  8. and S. Trinca. 1988. Induction of micronuclei
  9. in Vicia faba roots tips treated with heavy
  10. metals (cadmium and chromium) in the
  11. presence of NTA. Mutat. Res. 206, 311-315.
  12. Eckel, P.M. 1995. Aquatic genotoxicity testing with
  13. rat hepatocytes in primary culture II. Induction
  14. of micronuclei and chromosomal aberrations.
  15. Sci Total Environ. 159, 81-89.
  16. Egito L. C. M., M.G. Medeiros, S. R. B. Medeiros,
  17. and L.F. Agez-Lima. 2007. Cytotoxic and
  18. genotoxic potential of surface water from the
  19. Pitimbu river, northeastern /RN Brazil.
  20. Genetics and Molecular Biology, 30 (2), 435-
  22. Elezaj, I.R., K.H.Kurteshi, Q.I.Selimi, and
  23. K.Rr.Letaj. 2003. Genotoxic effects of Sitnica
  24. river (polluted from effluent waters of
  25. ââ‚‌œKosovaââ‚‌ Power plant) in three fish species
  26. (Barbus barbus, Perca fluviatilis and
  27. Oncorhynchus muciss Walbaum). FASEB.
  28. A1368/ 886.38.
  29. Haider, T., R. Sommer, S. Knasmuller, P. Eckel,
  30. W. Pribil, A. Cabaj and M. Kundi.2002.
  31. Genotoxic response of Austrian groundwater
  32. samples treated under standardized UV (254
  33. nm)- disinfection conditions in a comb ination
  34. of three different bioassays. Water Research.
  35. , 25-32.
  36. Helma C., S. Knasmuller , and R. Schulte Herman.
  37. Bie Belastung von Wassern mit
  38. genotoxischen Substanzen I. Methoden zur
  39. Prufung der Genotoxizita. Z. Umweltchem
  40. Okotox 6, 277-288.
  41. Ivanova, E., A. S. Teodora, and I. Velcheva. 2005.
  42. Cytogenetic testing of heavy metal and
  43. cyanide contaminated river waters in a mining
  44. region of Southwest Bulgaria. Journal of Cell
  45. and Molecular Biology. 4, 00-106.
  46. Knasmuller, S., E. Gottman, H. Steinkellner, A.
  47. Fomin, Pickl C, A. Paschke, R. God, and M.
  48. Kundi.1998. detection of genotoxic effects of
  49. heavy metal contamination soils with plant
  50. bioassays. Mutation Research., 2720; 000-000.
  51. Knasmuller, S., C. Helma, P. M. Eckel, E.
  52. Gottman, H. Steinkellner, F. Kassie, T. Haider,
  53. W.Parzefall, and R. Schulte-Hermann. 1998.
  54. Investigation on genotoxic effects of
  55. groundwater from the Mitterndorfer Senke and
  56. from the vicinity of Wiener Neustadt. 110, 23,
  57. -833.
  58. Kundi.1998. detection of genotoxic effects of heavy
  59. metal contamination soils with plant bioassays.
  60. Mutation Research., 2720;000-
  61. micronucleus (TradMCN) bioassay on
  62. clastogenicity of wastewater and in situ
  63. monitoring. Mutat. Res. 270, 45-51.
  64. Ma, T.H., 1982. Tradescantia cytogenetic tests
  65. (root-tip mitosis, pollen mitosis, pollen mother
  66. ââ‚‌“ cell meiosis). Mut. Res., 99, 293-302.
  67. Ma, T.H., Z. Xu, C. Xu, H. McConnell, E. V.
  68. Rabago, G.A. Arreola and H. Zang. 1995. The
  69. improved Allium/Vicia root tip micronucleus
  70. assay for clasto genicity of environmental
  71. pollutants. Mutation Research - Environmental
  72. Mutagenesis and related Subjects. 334 (2),
  73. -195.
  74. Majer, B., D. Tscherko, A. Paschke, R. Wennrich,
  75. M. Kundi, E. Kandellr and S. Knasmuller.
  76. Effects of heavy metal contamination of
  77. soils on micronucleus induction in Tradescantia
  78. and microbial enzyme activities: a
  79. comparative investigation. Mutation Research.
  80. , 111-124.
  81. Matsumoto, S.T., M.S. Mantovani, M. Irene, A.
  82. Malaguttii, A.L. Dias, I. C. Fonseca and M.A.
  83. Marin-Morales. 2006. Genotoxicity and
  84. mutagenicity of water contaminated with
  85. tannery effluents, as evaluated by
  86. micronucleus test and comet assay using fish
  87. Oreochromis niloticus and chromosome
  88. aberrations in onion root-tips. Genetics and
  89. Molecular Biology. 29 (1), 148-158
  90. Misk, M., T.H., Ma, A. Nersesyan, S. Monarca,
  91. J.K. Kim and S. Knasmuller. 2011.
  92. Micronucleus assays with the Tradescantia
  93. pollen tetrads: and update. Mutagenesis., 26
  94. (1), 215-221.
  95. Odeigah, P.G.C., O. Nurudeen, and O.O. Amund .
  96. Genotoxicity of oil field wastewater in
  97. Nigeria. Hereditas. 126, 161-167.
  98. Rizaj, M., Beqiri E, McBow, Oââ‚‌™Brein E.Z,
  99. Kongoli F, 2008. The mineral base and
  100. productive capacities of metals and nonmetals
  101. of Kosovo. JOM., vol.60 No.8:18-22.
  102. Ruiz, E. F., V. M. E. Rabago, S. U. Lecona, A. B.
  103. Perez and T. H. Ma. 1992. Tradescantia ââ‚‌“
  104. micronucleus (Trad-MCN) bioassay on clasto
  105. genicity of wastewater and in situ monitoring.
  106. Mutat. Res. 270, 45-51.
  107. Samka-Kinci, V., P. Stegnar, M. Lovka and M. J.
  108. Toman. 1996. The evaluation of waste,
  109. Surface and ground water quality using the
  110. Alium test procedure. Mutation Research/
  111. Genetic Toxicology. 368 (3-4), 171-179.
  112. Samuel , O.B., F.I. Osuala and P. G. C. Odeigah.
  113. Cytogenotoxicity evaluation of two
  114. industrial effluents using Allium cepa assay.
  115. African Journal of Environmental Science and
  116. Technology. 41 (1), 21-27.
  117. ZSandhu, S.S., T.H. MA, Y. Peng and H. Zhou.
  118. Clastogenicity of seven chemicals
  119. commonly found at industrial waste sites.
  120. Mutat. Res. 224, 437-445.
  121. Shokal R.R., and F.J. Rholf. 1981. Biometry, 2nd
  122. Edn., Freeman. New York, 1981, pp. 427-428.
  123. Steinkenller, H., F. Kassie, and S.
  124. Knasmuller.1999. Tradescantia ââ‚‌“ micronucleus
  125. assay for the assessment of the clastogenicity
  126. of Austrian water. Mutation Research. 426,
  127. -116.
  128. Tolbert, P. E., C. M. Shy, and J.W. Allen. 1992.
  129. Micronuclei and other ano- malies in buccal
  130. smears: methods Mutation Research, 271, 69-
  132. Zahn, R. K., H. C. Schroder, and H. G.
  133. Miltenburger. 1992
Statistics
Article View: 93
PDF Download: 26