Presence of Aflatoxin M1 in Pasteurized and UHT Milk Commercialized in Shiraz, Khuzestan and Yazd, Iran

Authors

1 Department of Food Hygiene, School of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, P.O.Box 166, Shahrekord, Iran

2 Graduated of Food Sciences and Technology, School of Agriculture, Shahrekord Branch, Islamic Azad University, P.O.Box 166, Shahrekord, Iran

Abstract

Aflatoxin M1 (AFM1) is a hydroxylated metabolite of the carcinogen aflatoxin B1 (AFB1), and may be found in the milk of lactating animals that have ingested feedstuffs contaminated with AFB1 Between Jun 2010 and March 2011, 149 samples of pasteurized (n=90) and UHT (n=59) milk were collected from supermarkets in the cities of Shiraz, Khuzestan and Yazed, Iran. All samples were analyzed for AFM1 contamination by ELISA and 142 (95.3%) were positive with mean concentrations 41 ng.l-1. These concentrations are lower than the standads of Codex Alimentarius and FDA (500 ng.l-1), but 33 samples (22.1%) had higher concentrations than the maximum tolerance accepted by some European countries (50 ng.l-1). Mean concentrations of AFM1 in pasteurized and UHT milk were 39 and 46 ng.l-1, respectively. Mean concentrations of AFM1 in fall and winter samples were significantly higher (P<0.05) than those of summer but differences between AFM1 concentrations of fall and winter samples were not significantly different. No significant differences in Concentrations of AFM1 in milk samples between Shiraz, Khuzestan and Yazd. Considering the results, this survey revealed a high frequency of AFM1 contamination in milk samples from central part of Iran.

Keywords


Alborzi, S., B. Pourabbas, M. Rashidi and B.
Astaneh, 2006. Aflatoxin M1
contamination Pasteurized milk in Shiraz
(South of Iran). Food Control, 17, 582-
Bakirci, I., 2001. A study on the occurrence of
aflatoxin M1 in milk and milk products
produced in Van province of Turkey.
Food Control, 12, 47-51.
Creppy, E.E., 2002. Update of survey, regulation
and toxic effects of mycotoxins in Europe.
Toxicol. Lett., 127, 19-28.
Codex Alimentarius Commission, 2001.
Comment submitted on the draft maximum
level for aflatoxin M1 in milk. In Codex
committee on food additives and
contaminants 33rd session. Hauge, The
Netherlands.
European Commission Regulation, No
/2001/EC of 8 March 2001, setting
maximum levels for certain contaminants in
food stuffs. Official Journal of the European
Commission Lo77: 2001; 1-13.
Fallah, A. A., 2010. Aflatoxin M1 contamination
in dairy products marketed in Iran during
winter and summer. Food Control, 21 ,
-1481.
FAO/WHO, 1999. Forty-ninth report of the joint
FAO/WHO Expert Committee of Food
Additives: Evaluation of Certain Food
Additive and Contaminations. WHO
Technical report Series, 884, 69-77.
Galvano, F., V. Galofaro and G. Galvona, 1996.
Occurrence and stability of aflatoxin M1 in
milk and milk products: a worldwide review.
J. Food Prot., 59, 1079-1090.
Hussain, I. and J. Anwar, 2008. A study on
contamination of aflatoxin M1 in raw milk in
the Punjab province of Pakistan. Food
Control, 19, 393-395.
International Agency for Research on Cancer,
Some Naturally Occurring Substances:
food items and constituents, heterocyclic
aromatic amines and mycotoxins.
Monographs on the Evaluation of
Carcinogenic Risks to Humans, 56, 245-395
(Lyon: IARC).
Ghanem, I. and M. Orfi, 2009. Aflatoxin M1 in
raw, pasteurized and powdered milk
available in Syrian market. Food Control,
, 603ââ‚‌“605.
Kamkar, A., 2005. A study on the contamination
of aflatoxin M1 in raw milk produced in
Sarab city of Iran. Food Control, 16, 593-
Karim, G., 1998. Study on the contamination of
raw bulk milk with aflatoxin M1 in Tehran
area using ELISA method (Persian). J.
Pajoohesh va Sazandeghi, 40-42, 163-165.
Lafont, P., M. Siriwardana and J. Lafont, 1989.
Genotoxicity of hydroxylaflatoxins M1 and
M4. Microbiol. Aliments Nutr., 7, 1-8.
Lopez, C.E., L.L. Ramos, S.S. Ramadan and L.C.
Bulacio, 2003. Presence of aflatoxin M1 in
milk for human consumption Argentina.
Food Control, 14, 31-34.
Movassagh, M.H., 2011. Presence of aflatoxin
M1 in UHT milk in Tabriz (northwest of
Iran). J. Food Safety, 31, 238ââ‚‌“241.
Oveisi, M.R., B. Jannat, N. Sadeghi, M.
Hajiohamoodi and A. Nikzad, 2007.
Presence of aflatoxin M1 in milk and infant
milk products in Tehran, Iran. Food Control,
, 1216-1218.
Park, D.L., 2002. Effect of processing on
aflatoxin. Adv. Exp. Med. Biol., 504, 173-
Rahimi, E., M. Bonyadian, M. Rafei, H.R.
Kazemeini, 2009a. Occurrence of aflatoxin
M1 in raw milk of five dairy species in
Ahvaz, Iran. Food Chem. Toxicol., 48, 129-
Rahimi, E., A. Shakerian, M. Jafariyan, M.
Ebrahimi and M. Riahi, 2009b. Occurrence
of aflatoxin M1 in raw, pasteurized and
UHT milk commercialized in Esfahan and
Shahr-e Kord, Iran. Food Security, 1, 317-
Saitanu, K., 1997. Incidence of aflatoxin M1 in
Thai milk products. J. Food Prot. 60, 1010ââ‚‌“
Stoloff, L., H.P. Van Egmond and D.L. Parks,
Rationales for the establishment of
limits and regulations for mycotoxins. Food
Addit. Contam., 8, 222-231.
Tajkarimi, M., F. Shojaee Alibadi, M. Salah
Nejad, H. Pursoltani, A.A. Motallebi and H.
Mahdavi, 2007. Seasonal study of aflatoxin
M1 contamination in milk in five regions in
Iran. Int. J. Food Microbiol., 116, 346-349.
Tajkarimi, M., F. Aliabadi-Sh, A. Salah Nejad,
H. Pursoltani, A.A. Motallebi and H.
Mahdavi, 2008. Aflatoxin M1 contamination
in winter and summer milk in 14 states in
Iran. Food Control, 19, 1033-1036.
Trucksess, M.W., 1999. Mycotoxins. Journal of
the AOAC International, 82, 488-495.
Unusan, N., 2006. Occurrence of aflatoxin M1 in
UHT milk in Turkey. Food Chem. Toxicol.,
, 1897ââ‚‌“1900.
Van Egmond, H.P., 1989. Mycotoxins in Dairy
Products. Elsevier Applied Science, London